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KMID : 0881720140290030234
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Journal of Food Hygiene and Safety
2014 Volume.29 No. 3 p.234 ~ p.240
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Development of an Analytical Method for Fluxapyroxad Determination in Agricultural Commodities by HPLC-UVD
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Kwon Ji-Eun
Kim Hee-Jeong
Park Hye-jin
Yoon Ji-Young
Lee Ji-Young
Chang Moon-Ik
Rhee Gyu-Sik
Do Jung-Ah
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Abstract
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Fluxapyroxad is classified as carboxamide fungicide that inhibits succinate dehydrogenase in complex II of mitochondrial respiratory chain, which results in inhibition of mycelial growth within the fungus target species. This study was carried out to assure the safety of fluxapyroxad residues in agricultural products by developing an official analytical method. A new, reliable analytical method was developed and validated using High Performance liquid Chromatograph- UV/visible detector (HPLC-UVD) for the determination of fluxapyroxad residues. The fluxapyroxad residues in samples were extracted with acetonitrile, partitioned with dichloromethane, and then purified with silica solid phase extraction (SPE) cartridge. Correlation coefficient(R2) of fluxapyroxad standard solution was 0.9999. The method was validated using apple, pear, peanut, pepper, hulled rice, potato, and soybean spiked with fluxapyroxad at 0.05 and 0.5 mg/kg. Average recoveries were 80.6~114.0% with relative standard deviation less than 10%, and limit of detection (LOD) and limit of quantification (LOQ) were 0.01 and 0.05 mg/kg, respectively. All validation parameters were followed with Codex guideline (CAC/GL 40). LC-MS (Liquid Chromatograph-Mass Spectrometer) was also applied to confirm the analytical method. Base on these results, this method was found to be appropriate fluxapyroxad residue determination and can be used as the official method of analysis.
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KEYWORD
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fluxapyroxad, HPLC-UVD, LC-MS, official method of analysis
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